Solution Information | help | |
Enzyme: | Lysine-specific demethylase 4E | |
inhibitor: | BDBM26114 | |
substrate: | Histone H3 fragment substrate | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.5 | |
Temp. Prep.: | 37 | |
Comments: | n/a | |
Solute 1: | FDH | |
Concentration: | 0.1 unit | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | Fe (II) | |
Concentration: | 10 uM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | 2-OG | |
Concentration: | Km | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | NAD+ | |
Concentration: | 500 uM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 2: | DMSO | |
Concentration: | 5% | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | HEPES | |
Concentration: | 50 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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